The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes.
Previous studies in mice showed that resistin impairs glucose tolerance and insulin action.
In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells.
Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (Direct and Indirect): 1:5000-1:10,000Western Blot: 1:2000-1:10,000 using ECL.
Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk.
Suggested incubation time is 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added. Labeled with Biotin.
Specificity：Recognizes human Resistin. Weakly cross-reacts with mouse Resistin. Detects a band of ~10kD by Western blot.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Resistin.
