The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes.
Previous studies in mice showed that resistin impairs glucose tolerance and insulin action.
In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells.
Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (Direct and Indirect): 1:5000-1:10,000 Western Blot: 1:2000-1:10,000 (ECL).
Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20, 5% skim milk; suggested incubation time is 1 hour at RTOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity：Recognizes human resistin at ~10kD. Weakly crossreacts with mouse resistin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human resistin.
