RELM-beta is a 19kD disulfide-linked homodimeric protein expressed in the epithelium of the colon and small bowel.
RELM-beta has been suggested to play a regulatory role during inflammation and may also act to establish links among adipose tissue, the intestine and the liver.
Interestingly, the molecular structure of RELM-beta is highly homologous to that of the adipose-derived cytokine resistin.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:5000-1:10,000Western Blot: 1:5000-1:10,000 using ECL.
Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk.
Suggested incubation time is 1 hour at room temperatureImmunohistochemistry: 5ug/mlOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human RELM-beta. Does not detect human Resistin, mouse Resistin, mouse RELM-α, mouse RELM-β or mouse RELM-γ. Detects a band of ~19kD by Western blot.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human RELM-beta.
