beta-PIX acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling.
It interacts with PAK kinases through the SH3 domain.
It may function as a positive regulator of apoptosis and is involved in cell migration.
It is expressed with high levels in the central nervous system.
Applications:Suitable for use in ELISA, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (Direct and Indirect): 1:5000-1:10,000Western Blot: 1:2000-1:5000 using ECL.
Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk.
Suggested incubation time is 1 hour at room temperatureImmunocytochemistry: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes the multiple splice variants of human, mouse and rat beta-Pix a, b, bL, c and d-isoforms. Cross-reacts with human and rat beta-Pix.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to mouse beta-Pix SH3 domain.
