PIDD promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis.
It associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-kappa-B.
It associates with caspase-2 and RAIDD, and induces activation of caspase-2 which is an important regulator in the apoptotic pathways.
Applications:Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: paraffin sectionsImmunoprecipitation: 1:100Western Blot: 1:500Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
Specificity：Recognizes human and the native form of mouse PIDD. Detects bands of ~90kD (full length protein) and ~50kD (cleaved protein) by Western blot.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human PIDD (Death Domain: aa776-910).
