NMNAT2 catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP.
Can also use the deamidated form nicotinic acid mononucleotide (NaMN) as a substrate but with lower efficiency.
Also catalyzes the reverse reaction, i.e.
the pyrophosphorolytic cleavage of NAD+.
Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen.
Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.
Has been shown to correlate with Alzheimer's disease in APPswe/PS1dE9 transgenic mice and delayed wallerian degeneration in cultured superior cervical ganglia (SCGs) from morphological changes, microtubule destruction and neurofilament degradation.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Immunohistochemistry: 15ug/mlWestern Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：guinea pig
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human NMNAT2. Detects a band of ~35kD by Western blot. Not tested in other species.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human NMNAT2.
