RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus.
MDA5, upon interaction with intracellular dsRNA (produced during viral replication), triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappaB, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5).
MDA5 is widely expressed at low levels.
The expression is detected at higher levels in placenta, pancreas and spleen and barely in brain, testis and lung.
Genetic variation in MDA5 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19).
A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: frozen sectionsWestern Blot: 1:1000Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes mouse MDA5.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa2-208 of mouse MDA5.
