Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis.
It is one of the most virulent foodborne pathogens.
Listeria species are known to secrete a 60kD protein collectively termed p60, which is encoded by the "iap" (invasion-associated-protein) gene and secreted in large quantites into the growth media.
p60 is a highly immunogenic murein hydrolase that is essential for cell division.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:5000-1:10,000Western Blot: 1:2000-1:10,000 using ECL.
Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk.
Suggested incubation time is 1 hour at room temperature
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes a wide range of Listeria p60 proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to Listeria spp. p60.
