IL-37 (Interleukin-37) belongs to the IL-1 family.
It binds to interleukin-18 receptor (IL-18R) but not to IL-1 receptor.
It was shown to play an important role as a link between innate and adaptive immunity and it is suggested as a new player in the inflammatory and immune responses mediated by the IL-18/IL-18R axis.
It was also shown to inhibit tumor growth.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA (Direct and Indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween-20, 5% skim milk; suggested incubation time is 1 hour at RTImmunohistochemistry: 1:50Immunoprecipitation: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity：Recognizes human IL-37. Detects a band of ~30kD by Western Blot.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IL-37.
