IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway.
Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues.
IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism.
Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Applications:Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (Direct and Indirect): 1:2000-1:10,000Western Blot: 1:2000-1:5000 using ECL; Suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; Suggested incubation time is 1 hour at RT
Flow Cytometry: 1:200-1:500Immunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human IDO. Detects a band of ~45kD by Western blot.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IDO.
