GITRL is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g., testis, ..).
GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies.
GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis.
Binding to GITR is important in regulating T-cell proliferation and TCR-mediated apoptosis.
GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (direct and indirect): 1:5000-1:10,000Western Blot: 1:2000-1:5000 using ECL.
Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk.
Suggested incubation time is 1 hour at room temperatureImmunohistochemistry: 5ug/mlOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human GITRL. Detects a band of ~15-17kD by Western blot. Does not cross-react with mouse GITRL.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human GITRL.
