FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse 'fused toes' mutation.
An association between FTO genotype and type 2 diabetes has been confirmed.
The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperatureImmunohistochemistry (paraffin sections): 1:500Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes mouse FTO. Detects a band of ~58kD by Western Blot. Weakly cross-reacts with human FTO.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse FTO.
