CTRP6 belongs to a highly conserved family of adiponectin paralogs.
CTRP6 mediates activation of AMP-activated protein kinase (AMPK) in skeletal muscle cells, thereby regulating glucose and lipid metabolism.
Mediates the phosphorylation of acetyl-CoA carboxylase (ACC).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:guinea pig
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity:Recognizes the N-terminal half of CTRP6 full-length protein. Detects bands of ~16kD and ~32kD by Western blot.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human CTRP6.