CTRP5 (C1qTNF-related protein 5; C1QTNF5) belongs to a highly conserved family of adiponectin paralogs.
CTRP5 mediates activation of AMP-activated protein kinase (AMPK) in muscle and liver cells, thereby regulating glucose and lipid metabolism.
Serum levels of CTRP5 are significantly higher in obese/diabetic animal models compared to normal controls.
Furthermore, CTRP5 may be a putative biomarker for mitochondrial dysfunction.
Defects in C1QTNF5 are a cause of late-onset retinal degeneration (LORD).
Applications:Suitable for use in Immunoprecipitation, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (Direct or Indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperatureImmunoprecipitation: 1:500-1:2000Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：guinea pig
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes a N-terminal region of CTRP5 full-length protein. Detects bands of ~15kD and ~26kD by Western blot. This antibody is CTRP5-specific.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human CTRP5.
