Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity.
It constitutes together with calnexin and ERp57 the 'calreticulin/calnexin cycle' that is responsible for folding and quality control of newly synthesized glycoproteins.
Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST, 0.05% Tween 20, 5% skim milk; suggested incubation time is 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human calreticulin. Detects a band of ~60kD by Western blot.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human calreticulin.
