ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced).
Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism.
Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes the coiled-coil domain of human ANGPTL4. Detects a band of ~19kD by Western blot. Weakly cross-reacts with human ANGPTL2 (coiled-coil domain), ANGPTL3 and ANGPTL5 (coiled-coil domain).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Coiled-coil domain of recombinant human ANGPTL4.
