ANGPTL3 regulates angiogenesis and also directly regulates lipid, glucose, and energy metabolism.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Immunohistochemistry: 5ug/mlWestern Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity:Recognizes the fibrinogen-like domain of human ANGPTL3. Detects a band of ~36kD by Western blot.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Fibrinogen-like domain of recombinant human ANGPTL3.