ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q.
Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation.
Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance.
Treatment of db/db mice with TZD increased ACRP30 levels.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:5000-1:10,000Western Blot: 1:5000-1:10,000 using ECL; suggested blocking and dilution buffer is TBST, 0.05% Tween 20, 5% skim milk; incubate 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human adiponectin. Detects a band of ~30kD by Western blot.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human adiponectin.
