84-1329-24 ID4, NT (ID4, BHLHB27, DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, Inhibitor of DNA binding 4) 200ul 036866
84-1329-24 ID4, NT (ID4, BHLHB27, DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, Inhibitor of DNA binding 4) 200ul 036866
ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems.
DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers.
Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain.
Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:10-50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human ID4. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human ID4. Species Sequence Homology: porcine
