SELT encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site.
The selenocysteine is encoded by the UGA codon that normally signals translation termination.
The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Applications:Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:Recognizes human SELT
Isotype:IgG
Calc Applications Abbrev:E FC WB
Calc Crossreactivity:Hu
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 48-75 in the N-terminal region of human SELT. Species Sequence Homology: bovine, chicken, mouse and rat