DFFA is a cell death process that removes toxic and/or useless cells during mammalian development.
The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units.
DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits.
DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis.
DFF becomes activated when DFFA is cleaved by caspase-3.
The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF.
DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：DFFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302~331 amino acids from the C-terminal region of human DFFA.
