FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution.
FGFR family members differ from one another in their ligand affinities and tissue distribution.
A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain.
The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation.
This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform.
Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations.
The genomic organization of the gene encompasses 20 exons.
Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity.
Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Indirect Flow Cytometry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000-1:2000Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:25Immunofluorescence: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human FGFR2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：FC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 22-51 in the N-terminal region of human FGFR2
