84-1322-55　E2F1, ID (E2F1, RBBP3, Transcription factor E2F1, PBR3, PRB-binding protein E2F-1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3) 200ul　034886

特徴

• E2F1 is a member of the E2F family of transcription factors.
• The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.
• The E2F proteins contain several evolutionally conserved domains found in most members of the family.
• These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.
• This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain.
• This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner.
• It can mediate both cell proliferation and p53-dependent/independent apoptosis.
• Applications:Suitable for use in Western Blot, Immunofluorescence, Indirect Flow Cytometry, ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500Immunofluorescence: 1:10-50Indirect Flow Cytometry: 1:10-50
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.