The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil.
In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin.
Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies.
The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors.
Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry: 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human AXIN2.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 816-843 in the C-terminal region of human AXIN2, UniProt Accession #Q9Y2T1. Species sequence homology: mouse, rat
