GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids.
It is a rate-limiting enzyme in the sialic acid biosynthetic pathway.
Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction.
Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells.
Applications:Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA.
Other applications have not tested.
Recommended Dilution:Western Blot: 1:1000Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human GNE. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 580-607 in the C-terminal region of human GNE. Swiss/UniProt Accession: Q9Y223. Species sequence homology: hamster, rat
