Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes.
The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.
This gene is intronless and encodes a member of the histone H2A family.
Transcripts from this gene lack polyA tails but instead contain a palindromic termination element.
This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Indirect Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be geterined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human HIST1H2AL at ~14kD.
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa103-130 in the C-terminal region of human HIST1H2AL, UniProt Accession #P0C0S8Cellular Localization: Nucleus, Chromosome. Species sequence homology: bovine, mouse and rat
