84-1312-80　H4 ( K20 [Me2] ) (HIST1H4A, H4/A, H4FA, Histone H4) 200ul　036410

特徴

• Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.
• The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA.
• A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.
• Applications:Suitable for use in Immunohistochemistry, Indirect Flow Cytometry, Western Blot, Dot Blot and ELISA.
• Other applications have not been tested.
• Recommended Dilutions:Dot Blot: 1:500Western Blot: 1:1000Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.