JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions.
In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300.
Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage.
In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments.
Activates the Arp2/3 complex to induce branched actin filament networks.
Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments.
Contributes to cell motility by controlling actin dynamics.
May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments.
The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：JMY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human JMY.
