Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane.
The activity of SNARE molecules is regulated by several soluble cytosolic proteins.
We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor.
GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity.
It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner.
We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:500Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human GABARAPL2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human GABARAPL2
