Side-chain deamidation of N-terminal asparagine residues to aspartate.
Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn.
These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH).
Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule.
This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position (By similarity).
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：NTAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the C-terminal region of human NTAN1.
