CD45 is a member of the protein tyrosine phosphatase (PTP) family.
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation.
This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP.
The CD45 gene is specifically expressed in hematopoietic cells.
This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.
It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.
This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:10-1:50Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human CD45.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1245-1275 in the C-terminal region of human CD45.
