Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit.
The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase.
The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic.
Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE).
Two transcript variants encoding the same protein have been found for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry, ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500Indirect Flow Cytometry: 1:10-50Immunofluorescence: 1:10-50Immunohistochemistry: 1:10-50Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human POLG.
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within aa1119-1148 from the C-terminal region of human POLG. Species sequence homology: mouse
