HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle.
Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site.
The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.
In addition, this protein regulates alternate splicing of the CFTR gene.
A similar pseudogene is pr[sent on chromosome 20.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:2000Immunohistochemistry (FFPE): 1:100Immunofluorescence: 1:10-1:50Optimal dilutions to be detrmined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human TARDBP at ~45kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa1-30 in the N-terminal region of human TARDBP. Cellular Localization: Nucleus. Species Sequence Homology: chicken
