LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain.
This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase.
It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3.
This protein may play a role in the repression of neuronal genes.
Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.
It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISAOther applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry: 1:50-100Immunofluorescence: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human LSD1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 819-852 in the C-terminal region of human LSD1, UniProt Accession #O60341. Species Sequence Homology: Mouse
