Covalent modification of histones plays critical role in regulating chromatin structure and transcription.
While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones.
A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function.
Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate.
Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9).
Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
Applications:Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：JHDM2b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 876-905 amino acids from the Central region of human JHDM2b.
