TRIM72 is a muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites.
Specifically binds phosphatidylserine.
Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site.
This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation.
Probably acts upstream of the Ca(2+)-dependent membrane resealing process.
Required for transport of DYSF to sites of cell injury during repair patch formation.
Regulates membrane budding and exocytosis.
May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:2000Immunohistochemistry (FFPE): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human TRIM72. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 299-327 in the C-terminal region of human TRIM72.
