Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli.
Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses.
Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation).
Binds also the PDGFR-alpha G-box promoter.
May have a role in modulating the cellular response to DNA damage.
Implicated in chromatin remodeling.
Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter.
Plays an essential role in the regulation of FE65 gene expression.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Immunofluorescence: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity：Recognizes human SP1
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 677-707 in the C-terminal region of human SP1. Species Sequence Homology: mouse and rat
