Melanoma antigen preferentially expressed in tumors is a protein that in humans is encoded by the PRAME gene.1,2,3 which encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes.
It is not expressed in normal tissues, except testis.
This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE.
However, unlike these other CT antigens, this gene is also expressed in acute leukemias.
Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:10-1:50Immunofluorescence: 1:10-1:50Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human PRAMEF6.
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 395-423 in the C-terminal region of human PRAMEF6, UniProt Accession #Q5VXH4; Q5TYX0; A6NMV5.
