Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis.
Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs.
Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA.
Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity).
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor.
Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7.
Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation.
Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells.
In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7.
Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7.
Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Mouse
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Mo
Immunogen：LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-147 amino acids from the Central region of human LIN28A.
