Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation.
It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel.
The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3.
The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8).
This gene encodes the d subunit of the Fo complex.
Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry and ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human ATP5H.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within between 68-97 amino acids from the central region of human ATP5H. Species sequence homology: bovine
