Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems.
The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids.
Our anti-Protein-C polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.
Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:Recognizes protein C tag.
Isotype:IgG
Calc Applications Abbrev:E WB
Immunogen:KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK)