Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques.
The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1).
The DYKDDDDK antibody binds to the same epitope recognized by anti-FLAG antibodies.
Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
