Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems.
The HA tag (YPYDVPDYAYPYDVPDYA) is an established example.
Our anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Applications:Suitable for use in Western Blot, Immunofluorescence, ChIP and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunofluorescence: 1:10-1:50ChIP: 1;100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:Recognizes Hemagglutinin Tag.
Isotype:IgG
Calc Applications Abbrev:E IF WB
Immunogen:KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA).