84-1239-20　Neuron Specific Enolase (NSE) 100ul　031410

特徴

• Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis.
• It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma.
• The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.
• NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells.
• ENO1 is also known as enolase alpha and as non-neuronal enolase.
• The third enolase, ENO3 or enolase beta, is expressed in muscle cells.
• Perhaps not surprisingly since neurons require a great deal of energy they are very rich in glycolytic enzymes such a GAPDH and NSE.
• Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells.
• Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).
• The HGNC name for this protein is ENO2.
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• A suitable control tissue is rat spinal cord or peripheral nerve homogenate.
• The NSE protein runs at about ~47kD on SDS-PAGE gels, and is a prominent component of brain, spinal cord and especially cortical extracts.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:500Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.