84-1239-18　Neurofilament M (Neurofilament Medium Polypeptide, NF-M, Neurofilament Triplet M Protein, 160kD Neurofilament Protein, Neurofilament 3, Nefm, Nef3, Nfm) 100ul　031408

特徴

• Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells.
• They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H.
• Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin.
• Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.
• This antibody was raised against the C-terminal region of NF-M, a region highly conserved between NF-M molecules in different species.
• Accordingly the antibody recognizes all mammalian and avian NF-M molecules.
• The name NF-M refers to the fact that this is the "middle" or "medium" protein of neurofilament triplet, the other two proteins being "low" or "light" (NF-L) and "high" or "heavy" (NF-H).
• NF-M is sometimes also referred to as NEF3 or Neurofilament 3.
• The HGNC name for this protein is NEFM.
• Applications:Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry (ABC): 1:5000Immunofluorescence: 1:500-1:1000Western Blot: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.