Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells.
They are composed a mixture of subunits which include the neurofilament triplet proteins, NF-L, NF-M and NF-H.
Neurofilaments may also include other intermediate filamant subunits such as α-internexin, peripherin and in developing neurons nestin and vimentin.
Antibodies to neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable and found only in neurons.
Mutations in the protein coding region of the human NF-L gene appear to cause some forms of Charcot-Marie-Tooth disease (2) The HGNC name for this protein is NEFL.
Applications:Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunocytochemistry (ABC): 1:5000Immunofluorescence: 1:500-1:1000Western Blot: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：porcine
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes porcine and rat NF-L.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Po Rt
Immunogen：Porcine intermediate filaments were prepared from spinal cords by the method of Delacourte et al., and this cytoskeletal material was dissolved in 6M urea.
