Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells.
They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H.
Neurofilaments may also include peripherin, a>-internexin, nestin and in some cases vimentin.
Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.
The form of NF-H expressed in neurons varies in terms of the level of phosphorylation.
Mammalian NF-H contains tandem repeats based on the sequence lysine serine proline (KSP), which may be present up to 60 times, with some variability between species.
These KSP sequences are the sites of phosphorylation, and in axons most if not all of the serine residues are phosphorylated.
In contrast, neurofilaments in dendrites and perikarya are not phosphorylated on these KSP sequences.
Axonal neurofilaments are therefore quite different immunologically from those in perikarya and dendrites, and many antibodies stain only axonal or only dendritic/perikaryal neurofilaments.
Since bacteria do not phosphorylate mammalian proteins, this protein resembles the NF-H form characteristic of perikaryal and dendritic neurofilaments.
Accordingly this antibody stains dendritic and perikaryal neurofilaments particularly well.
The HGNC name for this protein is NEFH.
Applications:Suitable for use in ELISA (detection antibody), Immunofluorescence Microscopy and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence Microscopy: 1:1000Western Blot: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes rat NF-H. Binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Rt
Immunogen：Purifed NF-H construct containing most of the tandem KSP repeats expressed in and purified from E. coli.
