84-1239-12　Microtubule Associated Protein 2 (MAP2, MAP-2) 50ul　031388

特徴

• Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells.
• They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit.
• Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.
• MAP2 is made up of two ~280kD apparent molecular weight bands referred to as MAP2a and MAP2b.
• A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kD on SDS-PAGE gels.
• All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau).
• MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells.
• Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
• In contrast t (tau) is found predominantly in neuronal axons.
• The HGNC name for this protein is MAP2.
• Applications:Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence (IF/IC): 1:10,000-1:20,000Western Blot: 1:20,000Immunohistochemistry: 1:5000-1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.