Matrix metalloproteinase-2 (MMP2) (gelatinase A, 72kD gelatinase, 72kD type IV collagenase), a member of the matrix metalloproteinase (MMP) enzyme family, is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis.
MMP2 degrades type IV collagen, the major structural component of basement membranes.
The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response.
Mutations in this gene have been associated with Winchester syndrome, Torg syndrome and nodulosis-arthropathy-osteolysis syndrome.
MMP2 is secreted as an inactive proenzyme/zymogen which is activated when cleaved by extracellular proteinases.
The proform has a molecular weight of 72kD and the active form 62kD.
A C-terminus peptide (aa475-490) was selected which is highly conserved and unique to MMP2, and is present in both pro- and active forms.
The peptide was conjugated to KLH and use to immunize rabbits.
The hyperimmune serum was affinity purified on a peptide-coupled sepharose column.
The purified immunoglobulin recognizes 72kD and 66kD bands corresponding to MMP2 on Western immunoblots.
Some tissue extracts also show MMP2 complexes and degradation products.
Polyclonal MMP2/475 antibody immunostains MMP2 specifically in various tissue preparations including paraffin sections.
The HGNC name for this protein is MMP2.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:1000Western Blot: 1:10,000; On Western Blots look for a major band at 66 and 72kD, corresponding to the proenzyme (72kD) and the activated form (66kD)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid.
Specificity：Recognizes MMP2. Species Crossreactivity: Human and rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Rt
Immunogen：Peptide corresponding to the C terminus of MMP2, specifically MGPLLVATFWPELPEK, which was coupled to keyhole limpet hemocyanin with glutaraldehyde
