Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered by as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epitethelial cells (1).
The MARCKS protein runs at about 80kD on SDS-PAGE gels, although the real molecular is much lower, at about 31.5kD.
The reason for this is probably because the protein is extremely rich in acidic residues.
Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein.
MARCKS is a major protein of brain, and is concentrated in synapses of neurons.
It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (2).
MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties (3).
Deletion of the MARCKS gene in mice results in embryonic brain defects and death (4).
The HGNC name for this protein is MARCKS.
Applications:Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunocytochemistry (ABC): 1:5000Immunofluorescence: 1:500-1:1000Western Blot: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes rat MARCKS.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Rt
Immunogen：Full length recombinant MARCKS expressed in and purified from E. coli.
